DNA barcoding Australia's fish species
Abstract
GC than sharks (44.7% versus 41.0%), again largely due to higher GC in the 3rd codon position in the former (36.3% versus 26.8%). Average within-species, genus, family, order and class Kimura two parameter (K2P) distances were 0.39%, 9.93%, 15.46%, 22.18% and 23.27%, respectively. All species could be differentiated by their cox1 sequence, although single individuals of each of two species had haplotypes characteristic of a congener. Although DNA barcoding aims to develop species identification systems, some phylogenetic signal was apparent in the data. In the neighbourjoining tree for all 754 sequences, four major clusters were apparent: chimaerids, rays, sharks and teleosts. Species within genera invariably clustered, and generally so did genera within families. Three taxonomic groups—dogfishes of the genus Squalus, flatheads of the family Platycephalidae, and tunas of the genus Thunnus—were examined more closely. The clades revealed after bootstrapping generally corresponded well with expectations. Individuals from operational taxonomic units designated as Squalus species B through F formed individual clades, supporting morphological evidence for each of these being separate species. We conclude that cox1 sequencing, or ‘barcoding’, can be used to identify fish species.
Cited in this thesis
Frequently Cited Together
- Minimally Invasive Evaluation of Venous Leg Ulcers in an Outpatient Setting Usin1 chapter
- Fish mislabelling in France: substitution rates and retail types1 chapter
- DeepSeek-R1: Incentivizing Reasoning Capability in LLMs via Reinforcement Learni1 chapter
- Application of rapid evaporative ionization mass spectrometry in preclinical and1 chapter
- Qualitative and quantitative analysis of adulterated Antarctic Krill Oil (AKO) b1 chapter
- DNA barcoding reveals mislabeling of endangered sharks sold as swordfish in New 1 chapter
BibTeX
@article{Ward2005,
author = {Robert D. Ward and Tyler S. Zemlak and B. H. Innes and Paul D. N. Hebert},
journal = {Philosophical Transactions of the Royal Society B},
title = {DNA barcoding Australia's fish species},
year = {2005},
abstract = {GC than sharks (44.7% versus 41.0%), again largely due to higher GC in the 3rd codon position in the former (36.3% versus 26.8%). Average within-species, genus, family, order and class Kimura two parameter (K2P) distances were 0.39%, 9.93%, 15.46%, 22.18% and 23.27%, respectively. All species could be differentiated by their cox1 sequence, although single individuals of each of two species had haplotypes characteristic of a congener. Although DNA barcoding aims to develop species identification systems, some phylogenetic signal was apparent in the data. In the neighbourjoining tree for all 754 sequences, four major clusters were apparent: chimaerids, rays, sharks and teleosts. Species within genera invariably clustered, and generally so did genera within families. Three taxonomic groups—dogfishes of the genus Squalus, flatheads of the family Platycephalidae, and tunas of the genus Thunnus—were examined more closely. The clades revealed after bootstrapping generally corresponded well with expectations. Individuals from operational taxonomic units designated as Squalus species B through F formed individual clades, supporting morphological evidence for each of these being separate species. We conclude that cox1 sequencing, or ‘barcoding’, can be used to identify fish species.},
doi = {10.1098/rstb.2005.1716},
mag_id = {2171405751},
pmcid = {1609232},
pmid = {16214743},
}